POSTERS

OP1

Segmental bone regeneration using vascular endothelial growth factor encapsulated poly D,L-Lactic Acid scaffolds and human bone marrow stromal cells
JM Kanczler*^[1], J Barry^[2], P Ginty^[2], SM Howdle^[2], KM Shakesheff^[2], ROC Oreffo^[1]
[1] Bone & Joint Group, University of Southampton, UK;^[2]School of Pharmacy & Chemistry, University of Nottingham, UK

OP2

CP55940, a non-selective CB1/CB2 agonist, stimulates bone resorption by human osteoclasts in vitro
L Whyte*, S Ridge, R Ross, MJ Rogers
Aberdeen OBRP Centre, Bone & Musculoskeletal Research Programme, University of Aberdeen, UK

OP3

Dietary flavonoid intake is associated with bone mineral density in early postmenopausal Scottish women
AC Hardcastle*^[1,2], JAM Kyle^[2], G Duthie^[3], G Mc Neill^[2], DM Reid^[1,2], HM MacDonald^[1,2]
[1]Osteoporosis Research Unit, Health Sciences Building, University of Aberdeen, UK;^[2]College of Medicine, University of Aberdeen, UK;^[3]Rowett Research Institute, Aberdeen, UK

OP4

Femoral neck microarchitecture: differences between cases of hip fracture and controls
N Loveridge*^[1], J Power^[1], H Kroger^[2], M Parker^[3], J Reeve^[1]
[1]Bone Research Group, Cambridge, UK;^[2]University Hospital, Kuopio, Finland;^[3]District Hospital, Peterborough, UK

OP5

Chondrocytes in osteoarthritis de-differentiate to a progenitor-like intermediate before re-differentiating to a complex and mixed phenotype
MA Da Silva, HI Roach*
Bone & Joint Research Group, University of Southampton, General Hospital, Southampton, UK

OP6

Abstract withdrawn
 

OP7

Osteoblasts protect multiple myeloma cells from T-cell-induced apoptosis
RM Locklin*^[1], RGG Russell^[1], PI Croucher^[2], CM Edwards^[3]
[1]Institute of Musculoskeletal Sciences, Botnar Research Centre,
Nuffield Department of Orthopaedic Surgery, University of Oxford,Oxford, UK;^[2]Division of Clinical Sciences, University of Sheffield Medical School, Sheffield, UK;^[3]Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, Tennessee, USA

OP8

The expression and regulation of Bim in osteoblasts undergoing growth factor withdrawal
M Liang*, RGG Russell, PA Hulley
Botnar Research Centre, Nuffield Dept of Orthopaedic Surgery, University of Oxford, UK

OP9

Novel control of GAG synthesis and chondrogenesis through selective regulation of UDPGD-mediated monosaccharide supply
CE Clarkin*, S Allen, BT Wheeler, CPD Wheeler-Jones, AA Pitsillides
The Royal Veterinary College, London, UK

OP10

The effect of tensile forces on the differentiation of mesenchymal stem cells
SB Mirza*,M Greenwood, G Blunn
Institute of Orthopaedics, University College London, UK

OP11

Abstract withdrawn

P12

Nitrogen-containing bisphosphonates and prediction of their clinical potencies: dissociation of target enzyme- and hydroxyapatite-binding affinities
Z Xia^[1]*, J Dunford^[1], MA Lawson^[1], JT Triffitt^[1], K Kavanagh^[1], U Oppermann^[1], BL Barnett ^[2], FH Ebetino^[2], RGG Russell^[1]
[1]The Oxford University Institute of Musculoskeletal Sciences, The Botnar Research Centre, Nuffield Orthopaedic Centre, Oxford,OX3 7LD, UK;^[2]New Drug Development, Procter and Gamble Pharm, Cincinnati OH, USA;^[3]Chemistry Department, University of Southern California, Los Angeles, CA, USA

P13

Mcl-1 is an important pro-survival factor in osteoclasts
K McConachie, D Tosh, MJ Rogers*
University of Aberdeen, Aberdeen, UK.

P14

Adenosine stimulates mineralisation of rat mesenchymal stem cells in vitro
B Gharibi*^[1,2], C Elford^[1], J Ham^[2], BAJ Evans^[1]
[1]Department of Child Health, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK;^[2]Centre for Endocrine and Diabetes Research, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK

P15

Compartmentalisation of GFP-tagged ER-alpha and ER-GR constructs in bone (ROS) and breast (MCF-7) cells
BS Maru*^[1], MR Norman^[1], JH Tobias^[2], CA McArdle^[1]
[1]Dorothy Hodgkin Building, University of Bristol, Whitson Street, Bristol, UK;^[2]Rheumatology Unit, Bristol Royal Infirmary, Marlborough Street, Bristol, UK

P16

Osteopenia in 129Sv/Ev Sparctm1cam mice: bone phenotypic characterization and gene expression changes
FC Mansergh^[1], T Wells^[1], C Elford^[2], SL Evans^[3], MJ Perry^[4], MJ Evans^[1], BAJ Evans*^[2]
[1]School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3US, UK;^[2]Department of Child Health, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK;^[3]School of Engineering, Cardiff University, The Parade, Cardiff CF24 3AA, UK;^[4]Department of Anatomy, University of Bristol, Bristol BS2 8EJ, UK

P17

SB431542, a TGF-beta type 1 receptor inhibitor, promotes C2C12 myotube hypertrophy
K Watt*, A Ratkevicius. H Wackerhage
Bone Research Group, Institute of Medical Sciences, University of Aberdeen, UK

P18

Polymorphic variation in the FPP synthase (FPPS) gene and effects on bisphosphonate inhibition
C Roemelt*^[1], F McGuigan^[2], J Dunford^[3], O Albagha^[4], MJ Rogers^[1]
[1]Bone & Musculoskeletal Research Programme, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK;^[2]Clinical & Molecular Osteoporosis Research Unit, Malmö University Hospital Malmö, Sweden SE-205 02;^[3]Structural Genomics Consortium, University of Oxford, Oxford OX3 7LD, UK;^[4]Rheumatology Section, Molecular Medicine Centre, University of Edinburgh School of Molecular and Clinical Medicine, Western General Hospital, Edinburgh, EH4 2XU, UK

P19

Human osteoclast formation and activity on bone substitute biomaterials
V Perrotti^[1,2], BM Nicholls*^[1], MA Horton^[1], A Piattelli^[2]
[1]University College London, Bone and Mineral Centre, London, UK;^[2]University of Chieti-Pescara, Dental School, Chieti, Italy

P20

Zoledronate potently inhibits the growth, survival and activity of normal rat osteoblasts
IR Orriss*, JC Utting, ML Key, A Brandao-Burch, KW Colston, TR Arnett
Department of Anatomy and Developmental Biology, University College London, London, UK

P21

Differential vascular endothelial growth factor receptor expression during osteogenesis
G Kirmizidis*^[1], JH Lakey^[2], MA Birch^[1]
[1]Musculoskeletal Research Group, Newcastle University, Newcastle upon Tyne, UK;^[2]Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK

P22

Bone formation and fibrocartilage regeneration at bone tendon junction with allogeneic cultured chondrocyte pellet interposition
MWN Wong*^[1], KO Tai^[1], KM Lee^[2], L Qin^[1], KS Leung^[1]
[1]Department of Orthopaedics and Traumatology;^[2]Lee Hysan Research Laboratory, The Chinese University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region of China

P23

Bcl-2-associated athanogene-1: A transcriptional regulator mediating chondrocyte survival and differentiation during endochondral ossification
RS Tare*^[1], PA Townsend^[2], GK Packham^[3], S Inglis^[1], ROC Oreffo^[1]
[1]Bone & Joint Research Group;^[2]Human Genetics;^[3]Cancer Sciences, University of Southampton, Southampton General Hospital, Southampton SO16 6YD, UK

P24

Nitric oxide regulates expression of Class 3 semaphorins in mouse osteoblasts
A Hughes*^[1], M Helfrich^[1], SH Ralston^[2], MJ Rogers^[1]
[1]Bone & Musculoskeletal Research Programme, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK;^[2]Molecular Medicine Centre, Western General Hospital, Crewe Road West, Edinburgh, EH4 2XU, UK

P25

Differential effects of alpha-halogenation on the potency of bisphosphonates and phosphonocarboxylates for inhibition of their target enzymes
CA Stewart*^[1], JE Dunford^[2], Z Xia^[2], R Baron^[3], MS Marma^[4], BA Kashemirov^[4], CE McKenna^[4], FH Ebetino^[5], FP Coxon^[1]
[1]Bone Research Group, Institute of Medical Sciences, University of Aberdeen, AB25 2ZD, UK;^[2]Botnar Research Centre, University of Oxford, UK;^[3]Imperial College, London, UK;^[4]Dept. of Chemistry, University of Southern California, Los Angeles, CA, USA;^[5]Procter & Gamble Pharmaceuticals, Cincinnati OH, USA

P26

Un-coupling of bone turnover markers following glucocorticoid therapy for exacerbations of inflammatory bowel disease
MH Kriel*^[1], CS Probert^[1], WD Fraser^[2], JH Tobias^[1]
[1]University of Bristol, Bristol, UK;^[2]University of Liverpool, Liverpool, UK

P27

Bone prophylaxis in steroid therapy: Are we doing enough?
T Dugal^[1],B Hameed*^[1],S Jawed^[2]
[1]St Georges Hospital Tooting, London;^[2]St Thomas's Hospital London;^[3]Kingston Hospital London

P28

The pharmacology and putative function of BK channels in human osteoblast-like cells
B Li*^[1], BAJ Evans^[2], KT Wann^[1]
[1]Welsh School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Ave, Cardiff CF10 3XF, UK;^[2]Department of Child Health, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN,UK

P29

Detection of acoustic emissions to assess press-fit stability and fracture propagation during the insertion of a simulated implant for cementless hip replacement.
BP Tweedie*, AY Muir, HA Simpson
Edinburgh Orthopaedic Engineering Centre, Chancellor's Building,
University of Edinburgh, UK

P30

Gas6 and Axl receptor tyrosine kinase are expressed by growth plate chondrocytes and osteoblasts
L Ronaldson*^[1,2], A Canfield^[2], C Farquharson^[1]
[1]Gene Function and Development, Roslin Institute, Roslin, Midlothian, UK;^[2]Cardiovascular and Endocrine Sciences, School of Medicine, University of Manchester, Manchester, UK

P31

The use of skeletal age to verify chronological age in youth football players
M Johnson*^[1], P Doherty^[2], AJ Freemont^[3]
[1]Manchester United Football Club, Manchester, UK;^[2]York St John University, York, UK^[3]Manchester University, Manchester, UK

P32

Extent of vitamin D insufficiency in young British women: influence on bone health
JL Berry^[1]*, DP Pattison^[2] AD Woolf^[2], SA Lanham-New^[3]
[1]University of Manchester, UK;^[2]University of Plymouth, UK;^[3]University of Surrey, UK

P33

Does hip arthritis limit the life expectancy in the elderly: A comparative study of operated and non-operated groups of patients suffering from hip osteoarthritis
SVM Karuppiah, A Dhaliwal, WM Ledingham
Department of Orthopaedics, Grampian University Hospitals Trust, Woodend Hospital, Eday Road Aberdeen AB25 2ZN, Scotland, UK

P34

The subcellular localisation of FEO-RANK is altered when co-expressed with wildtype RANK protein in vitro
JC Crockett*, M Helfrich, MJ Rogers
Bone Research Group, Institute of Medical Sciences, University of Aberdeen, UK

P35

Altered lipid content in osteoarthritic femurs, assessed using MRI
T Ahearn, JS Gregory*, TW Redpath, RM Aspden, JD Hutchison, S Semple, D Younie, D Knight, FJ Gilbert
University of Aberdeen, UK

P36

Changes in structural properties lead to reduced bone strength in Gunmetal mice
SR Goodyear*^[1,2], A Taylor^[1], FP Coxon^[1], IR Gibson[1,2], JMS Skakle^[2], RPK Wells^[2], RM Aspden^[1]
[1]Department of Orthopaedics, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK;^[2]Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen, AB24 3UE, UK

P37

Inter and Intra-observer repeatability of Kellgren-Lawrence grading for osteoarthritis using DXA images
CD Campbell*, JS Gregory, K Yoshida, N Basu, A Stewart, DM Reid, RM Aspden
Bone Research Programe, University of Aberdeen, UK

P38

Assessment of osteoporosis and osteoarthritis using active shape and active appearance models with DXA scans
JS Gregory*, RJ Barr, A Stewart, DM Reid, RM Aspden
Bone Research Programe, University of Aberdeen, UK

P39

Atypical pharmacology of P2X7 receptors in human osteoblasts
SM Al-qallaf*^[1,2], EJ Kidd^[1], BAJ Evans^[2]
[1]Welsh School of Pharmacy, Cardiff University, Cardiff, UK;^[2]Department of Child Health, School of Medicine, Cardiff University, Cardiff, UK

P40

eNOS knock out mice show reduced translocation of beta-Catenin to the nucleus when stimulated with pulsatile or oscillating fluid flow
C Huesa*^[1,2], A Hughes^[1], RM Aspden^[2], MH Helfrich^[1]
[1]Department of Medicine and Therapeutics, University of Aberdeen, Aberdeen, UK;^[2]Department of Orthopaedics, University of Aberdeen, Aberdeen, UK

P41

The role of p21CIP1/WAF1 in glucocorticoid induced growth retardation
HC Owen*^[1][2], SF Ahmed^[2], C Farquharson^[1]
[1]Bone Biology Group, Roslin Institute, Edinburgh, UK;^[2]Bone & Endocrine Research Group, Royal Hospital for Sick Children, Glasgow, UK

P42

Variation in Wnt-7a is unlikely to be a cause of familial Congenital Talipes Equinovarus
G Liu^[1], J Inglis^[1], A Cardy^[2], D Shaw^[1], S Sahota^[1], L Sharp^[3], Z Miedzybrodzka*^[1]
[1] Department of Medicine and Therapeutics, University of Aberdeen, Aberdeen, Scotland, UK;^[2]Department of Public Health, University of Aberdeen, Aberdeen, Scotland, UK;^[3]National Cancer Registry Ireland, Cork, Ireland

P43

Effects of ageing on cortical and trabecular bone in radius and tibia: a high-resolution pQCT study
S Kaptoge*^[1], N Dalzell^[1], N Morris^[2], B Koller^[3], P Ruegsegger^[3], A Berthier^[4], L Braak^[4], J Reeve^[1]
[1]Department of Medicine, University of Cambridge, Cambridge, UK;^[2]Hethersett Surgery, Norfolk, UK;^[3]Scanco, Zurich, Switzerland;^[4]MEDES, Toulouse, France

P44

Bone marrow stromal cells and biomimetic collagen-hydroxyapatite scaffolds for skeletal tissue engineering
JI Dawson*^[1], DA Wahl^[2], JT Czernuszka^[2], ROC Oreffo^[1]
[1]University of Southampton, Southhampton, UK;^[2]Oxford University, Oxford, UK

P45

A nouvelle method of application of tensile forces to mesenchymal stem cells
M Greenwood, SB Mirza*, G Blunn
Institute of Orthopaedics, University College London, London, UK

P46

Regulation of osteogenic marker gene expression by growth hormone in osteoblastic cells derived from human alveolar bone is donor age-dependent
MM Beloti*, GE Crippa, CR Cardoso, JS Silva, AL Ros
.University of Sao Paulo, Ribeirao Preto, SP, Brazil

P47

High Density Polyethylene as a substitute for bones in biomechanical studies
SV Karuppiah*^[1]' AJ Johnstone^[1]
[1]Robert Gordon University, Department of Engineering, Aberdeen, Scotland, UK

P48

Cortical and trabecular bone from mice compared by Raman spectroscopy
SR Goodyear*^[1,2], IR Gibson^[1,2], JMS Skakle^[2], RPK Wells^[2], RM Aspden^[1]
[1]Department of Orthopaedics, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK;^[2]Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen, AB24 3UE, UK

P49

The mechanical, material and chemical properties of cortical bone from nNOS null mice
SR Goodyear*^[1,3], RJ van't Hof^[2], IR Gibson^[1,3], JMS Skakle^[3], RPK Wells^[3], RM Aspden^[1]
[1]Department of Orthopaedics, Institute of Medical Sciences, Foresterhill, University of Aberdeen, Aberdeen AB25 2ZD, UK;^[2]Rheumatology Section, Molecular Medicine Centre, Western General Hospital, Edinburgh EH4 2XU, UK;^[3]Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen, AB24 3UE, UK

P50

The effects of zoledronate on iliac bone remodelling in stroke patients
KES Poole, N Loveridge, S Vedi, JE Compston, J Reeve*
Division of Bone Research, University of Cambridge, UK

P51

Serum markers of bone turnover: a novel approach to monitoring the natural history of Charcot Osteoarthropathy
C Moniz*, R Musto, N Petrova, M Edmunds, R Langworthy
Kings College Hospital, Denmark Hill, London SE5 9RS UK

P52

The use of statins in tissue engineering to enhance human bone cell culture
SL Griffiths*, SH Cartmell
Keele University, Institute of Science and Technology in Medicine, Guy Hilton Research Centre, Stoke, ST4 7QB, UK

P53

Cultured osteoblasts from osteoarthritic and osteoporotic patients display differential gene expression profiles
AJ Allstaff*, A Hughes, RM Aspden
Bone and Musculoskeletal Programme, University of Aberdeen, Aberdeen, UK

P54

Bone marrow quantification using 3.0 tesla magnetic resonance imaging
CP Bernard^[1], GP Liney*^[1], DJ Manton^[1], LW Turnbull^[1], CM Langton^[2]
[1]Centre for MR Investigations, Hull Royal Infirmary, UK; ^[2]Centre for Metabolic Bone Disease, Hull Royal Infirmary, UK

P55

Habitual physical activity and osteoarthritis: How can we best investigate whether there is a link between them?
S Sivakumar*, A Mavroeidi, DM Reid
University of Aberdeen, Aberdeen, UK

P56

Sample size requirements for bone density precision assessments and effect on patient misclassification: a Monte Carlo simulation study
A Moayyeri*^[1], M Sadatsafavi^[2], WD Leslie^[3]
[1]Department of Public Health and Primary Care, Institute of Public Health, School of Clinical Medicine, University of Cambridge, Cambridge, UK;^[2]Center for Clinical Epidemiology and Evaluation, Vancouver Coastal Health Institute, Vancouver, BC, Canada;^[3]Department of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada

P57

Wnt signalling in bone in osteoarthritis and osteoporosis
X Hochet, RM Aspden*
Department of Orthopaedics, University of Aberdeen, Scotland, UK

P58

Key determinants of bone mineral density in exercising young women
WWK To^[1]*, MWN Wong^[2]
[1] Dept of Obstetrics & Gynaecology, United Christian Hospital, Hong Kong, Hong Kong Special Administrative Region of China;^[2] Dept of Orthopaedics & Traumatology, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region of China

P59

Metal debris produced during total knee replacement: differences between metal and ceramic cutting jigs
S Wimsey*, AJ Langdown
Department of Orthopaedic Surgery, Queen Alexandra Hospital, Portsmouth, UK

P60

Lack of effect of alendronate therapy on circulating osteoclast precursor cell populations and their osteoclastogenic cytokine receptors
SJ Glover*, R Eastell, A Rogers
University of Sheffield, Sheffield, UK

P61

Tumour cell-bone marrow stromal cell interactions modify expression of Cathepsin K, ADAMTS-15, TIMP-3 and osteoprotegerin (OPG)
JKL Woodward*, DV Lefley, NA Cross, PD Ottewell, DJ Buttle, RE Coleman, I Holen
University of Sheffield, UK

P62

A functional RNAi screening for Runx2-regulated genes corresponding to ectopic bone formation in human spinal ligaments
M Kishiya*^[1,2], H Kudo^[2], K Kanemaru^[2], T Numasawa^[2], T Yokoyama^[2], S Motomura^[1], S Toh^[2], K-I Furukawa^[1]
[1]Department of Pharmacology, Hirosaki University School of Medicine, Hirosaki, Japan;^[2]Department of Orthopedic Surgery, Hirosaki University School of Medicine, Hirosaki, Japan

P63

The pattern of procalcitonin in uncomplicated total hip and knee arthroplasty and its implication in periprosthetic infection
SM Ali*, A Christ, A Chappell
Inverclyde Royal Hospital, Greenock, UK

P64

The importance of parathyroid hormone (PTH) assessment in the treatment of autosomal dominant hypophosphataemic rickets
N Basu*, AJ Black, DM Reid
Grampian Osteoporosis Service, Department of Rheumatology, Aberdeen Royal Infirmary, Aberdeen, Scotland